Luận án Nghiên cứu tạo cây đậu tương (Glycine Max L.) biến đổi gen có khả năng tổng hợp Astaxanthin chuyên biệt ở hạt

Nội dung text: Luận án Nghiên cứu tạo cây đậu tương (Glycine Max L.) biến đổi gen có khả năng tổng hợp Astaxanthin chuyên biệt ở hạt

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6. Javel 50m 28.60919* 5.30899 .000 16.7800 40.4384 Clo 24h Clo 16h -18.76498* 5.30899 .005 -30.5941 -6.9358 Javel 30m -13.97002* 5.30899 .025 -25.7992 -2.1408 Javel 40m -7.33776 5.30899 .197 -19.1669 4.4914 Javel 50m 9.84421 5.30899 .093 -1.9850 21.6734 Javel Clo 16h -4.79496 5.30899 .388 -16.6241 7.0342 30m Clo 24h 13.97002* 5.30899 .025 2.1408 25.7992 Javel 40m 6.63226 5.30899 .240 -5.1969 18.4614 Javel 50m 23.81423* 5.30899 .001 11.9851 35.6434 Javel Clo 16h -11.42722 5.30899 .057 -23.2564 .4019 40m Clo 24h 7.33776 5.30899 .197 -4.4914 19.1669 Javel 30m -6.63226 5.30899 .240 -18.4614 5.1969 Javel 50m 17.18198* 5.30899 .009 5.3528 29.0111 Javel Clo 16h -28.60919* 5.30899 .000 -40.4384 -16.7800 50m Clo 24h -9.84421 5.30899 .093 -21.6734 1.9850 Javel 30m -23.81423* 5.30899 .001 -35.6434 -11.9851 Javel 40m -17.18198* 5.30899 .009 -29.0111 -5.3528 *. The mean difference is significant at the 0.05 level. Bảng 3.5. Kết quả ảnh hƣởng của các nồng độ BA lên sự tái sinh của đốt lá mầm và một nửa hạt giống MTĐ 176 (Tỉ lệ mẫu tạo chồi đã đƣợc chuyển đổi sang dạng arcsin√x; Số chồi/mẫu đƣợc chuyển sang dạng log(x+1) trƣớc khi xử lý thống kê) MTTS1 Duncan Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 9.0974 0.5 mg/l 3 35.2178 1 mg/l 3 39.1475 1.5 mg/l 3 52.7753 3 mg/l 3 61.2197 2.5 mg/l 3 71.5651 2 mg/l 3 74.6776 Sig. 1.000 .229 1.000 1.000 .337 Means for groups in homogeneous subsets are displayed.
7. Bảng 3.6. Kết quả ảnh hƣởng của các nồng độ BA lên sự tái sinh của đốt lá mầm và một nửa hạt giống HL 07-15 (Tỉ lệ mẫu tạo chồi đã đƣợc chuyển đổi sang dạng arcsin√x; Số chồi/mẫu đƣợc chuyển sang dạng log(x+1) trƣớc khi xử lý thống kê) Duncan HLTS1 Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 9.0974 0.5 mg/l 3 37.2247 1 mg/l 3 46.9228 1.5 mg/l 3 61.2197 3 mg/l 3 74.6776 2 mg/l 3 77.7901 2.5 mg/l 3 77.7901 Sig. 1.000 1.000 1.000 1.000 .407 Means for groups in homogeneous subsets are displayed. Duncan HLSC1 Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 .0000 0.5 mg/l 3 .2333 1 mg/l 3 .4100 1.5 mg/l 3 .5733 3 mg/l 3 .7400 2.5 mg/l 3 .7700 2 mg/l 3 .7867 Sig. 1.000 1.000 1.000 1.000 .154 Means for groups in homogeneous subsets are displayed. HLTS2 Duncan Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 9.0974 0.5 mg/l 3 39.2315 1 mg/l 3 48.8457 1.5 mg/l 3 59.0044 2.5 mg/l 3 74.6776 2 mg/l 3 77.7901 3 mg/l 3 77.7901 Sig. 1.000 1.000 1.000 1.000 .383
8. Duncan OMSC1 Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 .0000 0.5 mg/l 3 .1933 1 mg/l 3 .3833 1.5 mg/l 3 .5833 3 mg/l 3 .7167 2.5 mg/l 3 .7533 2 mg/l 3 .7800 Sig. 1.000 1.000 1.000 1.000 .066 Means for groups in homogeneous subsets are displayed. OMTS2 Duncan Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 9.0974 0.5 mg/l 3 39.2315 1 mg/l 3 52.7753 1.5 mg/l 3 61.2197 2.5 mg/l 3 74.6776 2 mg/l 3 77.7901 3 mg/l 3 77.7901 Sig. 1.000 1.000 1.000 1.000 .385 Means for groups in homogeneous subsets are displayed. OMSC2 Duncan Subset for alpha = 0.05 NongdoBA N 1 2 3 4 5 0 mg/l 3 .0000 0.5 mg/l 3 .2200 1 mg/l 3 .4233 1.5 mg/l 3 .5933 3 mg/l 3 .7533 2.5 mg/l 3 .7633 2 mg/l 3 .7867 Sig. 1.000 1.000 1.000 1.000 .295 Means for groups in homogeneous subsets are displayed.
9. 1.5 mg/l .10000* .03113 .009 .0306 .1694 2 mg/l .20333* .03113 .000 .1340 .2727 1.5 mg/l 0 mg/l .38000* .03113 .000 .3106 .4494 0.5 mg/l .18333* .03113 .000 .1140 .2527 1 mg/l -.10000* .03113 .009 -.1694 -.0306 2 mg/l .10333* .03113 .008 .0340 .1727 2 mg/l 0 mg/l .27667* .03113 .000 .2073 .3460 0.5 mg/l .08000* .03113 .028 .0106 .1494 1 mg/l -.20333* .03113 .000 -.2727 -.1340 1.5 mg/l -.10333* .03113 .008 -.1727 -.0340 OMTR 0 mg/l 0.5 mg/l -7.69131* 3.42543 .049 -15.3236 -.0590 1 mg/l -17.85003* 3.42543 .000 -25.4824 -10.2177 1.5 mg/l -36.63571* 3.42543 .000 -44.2681 -29.0034 2 mg/l -27.70067* 3.42543 .000 -35.3330 -20.0683 0.5 mg/l 0 mg/l 7.69131* 3.42543 .049 .0590 15.3236 1 mg/l -10.15872* 3.42543 .014 -17.7911 -2.5264 1.5 mg/l -28.94441* 3.42543 .000 -36.5767 -21.3121 2 mg/l -20.00936* 3.42543 .000 -27.6417 -12.3770 1 mg/l 0 mg/l 17.85003* 3.42543 .000 10.2177 25.4824 0.5 mg/l 10.15872* 3.42543 .014 2.5264 17.7911 1.5 mg/l -18.78568* 3.42543 .000 -26.4180 -11.1533 2 mg/l -9.85064* 3.42543 .017 -17.4830 -2.2183 1.5 mg/l 0 mg/l 36.63571* 3.42543 .000 29.0034 44.2681 0.5 mg/l 28.94441* 3.42543 .000 21.3121 36.5767 1 mg/l 18.78568* 3.42543 .000 11.1533 26.4180 2 mg/l 8.93504* 3.42543 .026 1.3027 16.5674 2 mg/l 0 mg/l 27.70067* 3.42543 .000 20.0683 35.3330 0.5 mg/l 20.00936* 3.42543 .000 12.3770 27.6417 1 mg/l 9.85064* 3.42543 .017 2.2183 17.4830 1.5 mg/l -8.93504* 3.42543 .026 -16.5674 -1.3027 OMSR 0 mg/l 0.5 mg/l -.17000* .03026 .000 -.2374 -.1026 1 mg/l -.32667* .03026 .000 -.3941 -.2592 1.5 mg/l -.54000* .03026 .000 -.6074 -.4726 2 mg/l -.40667* .03026 .000 -.4741 -.3392 0.5 mg/l 0 mg/l .17000* .03026 .000 .1026 .2374 1 mg/l -.15667* .03026 .000 -.2241 -.0892 1.5 mg/l -.37000* .03026 .000 -.4374 -.3026 2 mg/l -.23667* .03026 .000 -.3041 -.1692 1 mg/l 0 mg/l .32667* .03026 .000 .2592 .3941 0.5 mg/l .15667* .03026 .000 .0892 .2241
10. 1.5 mg/l .13667* .03553 .003 .0575 .2158 2 mg/l .28333* .03553 .000 .2042 .3625 1.5 mg/l 0 mg/l .33333* .03553 .000 .2542 .4125 0.5 mg/l .07333 .03553 .066 -.0058 .1525 1 mg/l -.13667* .03553 .003 -.2158 -.0575 2 mg/l .14667* .03553 .002 .0675 .2258 2 mg/l 0 mg/l .18667* .03553 .000 .1075 .2658 0.5 mg/l -.07333 .03553 .066 -.1525 .0058 1 mg/l -.28333* .03553 .000 -.3625 -.2042 1.5 mg/l -.14667* .03553 .002 -.2258 -.0675 *. The mean difference is significant at the 0.05 level. Bảng 3.9. Ảnh hƣởng PPT lên sự tái sinh chồi của đốt lá mầm (Số liệu đã đƣợc chuyển đổi sang dạng arcsin√x trƣớc khi xử lý thống kê) MTD176 Duncan Subset for alpha = 0.05 NongdoPPT N 1 2 3 4 6 mg/l 3 9.0974 7 mg/l 3 9.0974 8 mg/l 3 9.0974 5 mg/l 3 21.1450 21.1450 4 mg/l 3 30.9956 2 mg/l 3 48.8457 3 mg/l 3 55.0447 1 mg/l 3 59.0044 59.0044 0 mg/l 3 71.5651 Sig. .105 .148 .156 .070 Means for groups in homogeneous subsets are displayed.
11. Bảng 3.10. Ảnh hƣởng của PPT lên sự sinh trƣởng đoạn thân (Số liệu đã đƣợc chuyển đổi sang dạng arcsin√x trƣớc khi xử lý thống kê) Multiple Comparisons 95% Confidence Interval (I) (J) Nongdo Nongdo Mean Difference Lower Upper Dependent Variable PPT PPT (I-J) Std. Error Sig. Bound Bound HL0715 LSD 0 mg/l 1 mg/l 5.82254 3.38843 .111 -1.5602 13.2053 2 mg/l 31.60038* 3.38843 .000 24.2176 38.9831 3 mg/l 39.45977* 3.38843 .000 32.0770 46.8425 4 mg/l 59.35511* 3.38843 .000 51.9723 66.7379 5 mg/l 65.58012* 3.38843 .000 58.1974 72.9629 1 mg/l 0 mg/l -5.82254 3.38843 .111 -13.2053 1.5602 2 mg/l 25.77784* 3.38843 .000 18.3951 33.1606 3 mg/l 33.63724* 3.38843 .000 26.2545 41.0200 4 mg/l 53.53257* 3.38843 .000 46.1498 60.9153 5 mg/l 59.75758* 3.38843 .000 52.3748 67.1403 2 mg/l 0 mg/l -31.60038* 3.38843 .000 -38.9831 -24.2176 1 mg/l -25.77784* 3.38843 .000 -33.1606 -18.3951 3 mg/l 7.85939* 3.38843 .039 .4766 15.2422 4 mg/l 27.75473* 3.38843 .000 20.3720 35.1375 5 mg/l 33.97974* 3.38843 .000 26.5970 41.3625 3 mg/l 0 mg/l -39.45977* 3.38843 .000 -46.8425 -32.0770 1 mg/l -33.63724* 3.38843 .000 -41.0200 -26.2545 2 mg/l -7.85939* 3.38843 .039 -15.2422 -.4766 4 mg/l 19.89534* 3.38843 .000 12.5126 27.2781 5 mg/l 26.12034* 3.38843 .000 18.7376 33.5031 4 mg/l 0 mg/l -59.35511* 3.38843 .000 -66.7379 -51.9723 1 mg/l -53.53257* 3.38843 .000 -60.9153 -46.1498 2 mg/l -27.75473* 3.38843 .000 -35.1375 -20.3720 3 mg/l -19.89534* 3.38843 .000 -27.2781 -12.5126 5 mg/l 6.22501 3.38843 .091 -1.1578 13.6078 5 mg/l 0 mg/l -65.58012* 3.38843 .000 -72.9629 -58.1974
12. 2 mg/l 36.63571* 3.18418 .000 29.6980 43.5734 3 mg/l 44.57915* 3.18418 .000 37.6414 51.5169 4 mg/l 62.46762* 3.18418 .000 55.5299 69.4053 5 mg/l 68.69262* 3.18418 .000 61.7549 75.6303 1 mg/l 0 mg/l -8.93504* 3.18418 .016 -15.8728 -1.9973 2 mg/l 27.70067* 3.18418 .000 20.7629 34.6384 3 mg/l 35.64411* 3.18418 .000 28.7064 42.5818 4 mg/l 53.53257* 3.18418 .000 46.5949 60.4703 5 mg/l 59.75758* 3.18418 .000 52.8199 66.6953 2 mg/l 0 mg/l -36.63571* 3.18418 .000 -43.5734 -29.6980 1 mg/l -27.70067* 3.18418 .000 -34.6384 -20.7629 3 mg/l 7.94344* 3.18418 .028 1.0057 14.8812 4 mg/l 25.83190* 3.18418 .000 18.8942 32.7696 5 mg/l 32.05691* 3.18418 .000 25.1192 38.9946 3 mg/l 0 mg/l -44.57915* 3.18418 .000 -51.5169 -37.6414 1 mg/l -35.64411* 3.18418 .000 -42.5818 -28.7064 2 mg/l -7.94344* 3.18418 .028 -14.8812 -1.0057 4 mg/l 17.88847* 3.18418 .000 10.9507 24.8262 5 mg/l 24.11347* 3.18418 .000 17.1758 31.0512 4 mg/l 0 mg/l -62.46762* 3.18418 .000 -69.4053 -55.5299 1 mg/l -53.53257* 3.18418 .000 -60.4703 -46.5949 2 mg/l -25.83190* 3.18418 .000 -32.7696 -18.8942 3 mg/l -17.88847* 3.18418 .000 -24.8262 -10.9507 5 mg/l 6.22501 3.18418 .074 -.7127 13.1627 5 mg/l 0 mg/l -68.69262* 3.18418 .000 -75.6303 -61.7549 1 mg/l -59.75758* 3.18418 .000 -66.6953 -52.8199 2 mg/l -32.05691* 3.18418 .000 -38.9946 -25.1192 3 mg/l -24.11347* 3.18418 .000 -31.0512 -17.1758 4 mg/l -6.22501 3.18418 .074 -13.1627 .7127 *. The mean difference is significant at the 0.05 level.
13. 2 lan 3 74.6776 0 lan 3 77.7901 Sig. 1.000 1.000 .244 Means for groups in homogeneous subsets are displayed. ảng 3 12 Tỉ lệ mẫu dƣơng tính GUS khi xử l sóng siêu âm ở các thời gian khác nhau (Số liệu đã đƣợc chuyển đổi sang dạng arcsin√x trƣớc khi xử lý thống kê). Multiple Comparisons Dependent Variable:GUSsieuam (I) (J) 95% Confidence Interval Thoigia Thoigia Mean Difference n n (I-J) Std. Error Sig. Lower Bound Upper Bound LSD 0s 15s -19.97912* 2.78926 .000 -26.1940 -13.7643 30s -32.02667* 2.78926 .000 -38.2415 -25.8118 45s -32.02667* 2.78926 .000 -38.2415 -25.8118 60s -32.02667* 2.78926 .000 -38.2415 -25.8118 15s 0s 19.97912* 2.78926 .000 13.7643 26.1940 30s -12.04755* 2.78926 .002 -18.2624 -5.8327 45s -12.04755* 2.78926 .002 -18.2624 -5.8327 60s -12.04755* 2.78926 .002 -18.2624 -5.8327 30s 0s 32.02667* 2.78926 .000 25.8118 38.2415 15s 12.04755* 2.78926 .002 5.8327 18.2624 45s .00000 2.78926 1.000 -6.2149 6.2149 60s .00000 2.78926 1.000 -6.2149 6.2149 45s 0s 32.02667* 2.78926 .000 25.8118 38.2415 15s 12.04755* 2.78926 .002 5.8327 18.2624 30s .00000 2.78926 1.000 -6.2149 6.2149 60s .00000 2.78926 1.000 -6.2149 6.2149 60s 0s 32.02667* 2.78926 .000 25.8118 38.2415 15s 12.04755* 2.78926 .002 5.8327 18.2624 30s .00000 2.78926 1.000 -6.2149 6.2149 45s .00000 2.78926 1.000 -6.2149 6.2149 *. The mean difference is significant at the 0.05 level.